The Mouse LEPR (Leptin Receptor) CLIA Kit is a specialized assay kit designed for the accurate measurement of leptin receptor levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring dependable and consistent results for various research applications.The leptin receptor is a key protein involved in regulating energy balance and metabolism, playing a crucial role in obesity, diabetes, and other metabolic disorders.
By accurately measuring leptin receptor levels, researchers can gain valuable insights into these conditions and potentially develop new therapeutic interventions.Overall, the Mouse LEPR CLIA Kit is a valuable tool for studying the role of the leptin receptor in metabolic diseases and advancing our understanding of key physiological processes.
Product Name:
Mouse LEPR (Leptin Receptor) CLIA Kit
SKU:
MOES00423
Target:
Mouse LEPR (Leptin Receptor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse LEPR. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse LEPR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse LEPR, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse LEPR. You can calculate the concentration of Mouse LEPR in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-102
90-105
87-100
Average (%)
94
98
94
1:4
Range (%)
87-98
84-97
95-111
Average (%)
93
91
102
1:8
Range (%)
92-106
87-100
92-103
Average (%)
99
94
97
1:16
Range (%)
100-114
88-100
93-108
Average (%)
105
94
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-101
93
EDTA plasma (n=5)
87-99
92
Cell culture media (n=5)
86-101
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
37.09
125.69
280.07
35.25
136.75
271.96
Standard deviation
3.89
13.9
21.82
4.43
14.54
21.02
C V (%)
10.49
11.06
7.79
12.57
10.63
7.73
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse LEPR concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse LEPR in samples. No significant cross-reactivity or interference between Mouse LEPR and analogues was observed.
