The Mouse LH (Luteinizing Hormone) CLIA Kit is a highly sensitive and specific assay designed for the accurate measurement of LH levels in mouse serum, plasma, and other biological samples. This kit offers reliable and reproducible results, making it ideal for various research applications.Luteinizing Hormone is a key hormone involved in the regulation of the reproductive system, specifically in the ovulation process in females and testosterone production in males.
Monitoring LH levels is crucial in understanding reproductive disorders, menstrual irregularities, and infertility.With its high performance and precision, the Mouse LH CLIA Kit is a valuable tool for researchers studying reproductive biology, endocrinology, and fertility issues in rodent models. Its ease of use and accuracy make it an essential component in investigating the effects of hormonal imbalances and potential therapeutic interventions.
Product Name:
Mouse LH (Luteinizing Hormone) CLIA Kit
SKU:
MOES00051
Target:
Mouse LH (Luteinizing Hormone)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.19 mIU/mL
Detection range:
0.31-20 mIU/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse LH. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse LH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse LH, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse LH. You can calculate the concentration of Mouse LH in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-103
95-109
103-117
Average (%)
98
101
109
1:4
Range (%)
94-111
88-100
96-112
Average (%)
102
95
102
1:8
Range (%)
102-118
89-102
92-108
Average (%)
107
94
98
1:16
Range (%)
100-115
83-97
92-104
Average (%)
106
90
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-109
102
EDTA plasma (n=5)
87-100
94
Cell culture media (n=5)
93-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.96
2.37
7.49
0.87
2.25
7.84
Standard deviation
0.08
0.19
0.68
0.07
0.24
0.61
C V (%)
8.33
8.02
9.08
8.05
10.67
7.78
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse LH concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse LH in samples. No significant cross-reactivity or interference between Mouse LH and analogues was observed.
