The Mouse LIFR (Leukemia Inhibitory Factor Receptor) CLIA Kit is specifically designed for the precise measurement of LIFR levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it ideal for a wide range of research applications.LIFR is a key receptor involved in the signaling pathway of Leukemia Inhibitory Factor (LIF), playing a crucial role in various biological processes such as cell differentiation, proliferation, and survival.
Dysregulation of LIFR has been linked to several diseases, including cancer and inflammatory disorders, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.With the Mouse LIFR CLIA Kit, researchers can confidently analyze LIFR levels in mouse samples with precision, providing valuable insights into the mechanisms underlying disease pathology and potential treatment strategies.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse LIFR. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse LIFR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse LIFR, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse LIFR. You can calculate the concentration of Mouse LIFR in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-111
91-107
95-107
Average (%)
104
98
101
1:4
Range (%)
100-116
99-110
96-111
Average (%)
109
104
102
1:8
Range (%)
85-98
98-111
98-115
Average (%)
92
105
105
1:16
Range (%)
91-105
86-98
93-104
Average (%)
97
93
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-103
97
EDTA plasma (n=5)
86-101
93
Cell culture media (n=5)
88-105
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
40.18
82.04
315.12
43.85
87.0
309.15
Standard deviation
4.74
7.29
20.36
4.0
7.37
28.6
C V (%)
11.8
8.89
6.46
9.12
8.47
9.25
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse LIFR concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse LIFR in samples. No significant cross-reactivity or interference between Mouse LIFR and analogues was observed.
