Description
Mouse Low affinity immunoglobulin gamma Fc region receptor II (Fcgr2) ELISA Kit
The Mouse Low Affinity Immunoglobulin Gamma Fc Region Receptor II (FCGR2) ELISA Kit is a cutting-edge tool designed for the accurate quantification of FCGR2 levels in mouse serum, plasma, and cell culture supernatants. This kit offers unparalleled sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.FCGR2 is a vital receptor involved in the immune response, specifically in the recognition and binding of antibodies. Its role in immune regulation and modulation makes it a key player in autoimmune diseases, infections, and inflammatory conditions.
By accurately measuring FCGR2 levels, researchers can gain valuable insights into immune system function and the development of potential therapeutic interventions.With its reliable performance and ease of use, the Mouse Low Affinity Immunoglobulin Gamma Fc Region Receptor II (FCGR2) ELISA Kit is a crucial tool for advancing research in immunology, inflammation, and autoimmune disorders. Get your kit today and unlock new discoveries in the field of immunology.
| Product Name: | Mouse Low affinity immunoglobulin gamma Fc region receptor II (Fcgr2) ELISA Kit |
| SKU: | MOEB1534 |
| Size: | 96T |
| Target: | Mouse Low affinity immunoglobulin gamma Fc region receptor II (Fcgr2) |
| Synonyms: | Fc gamma receptor IIB, IgG Fc receptor II beta, Lymphocyte antigen 17, Fc-gamma RII, Ly-17, CD32, Fcgr2b, Ly-17 |
| Assay Type: | Sandwich |
| Detection Method: | ELISA |
| Reactivity: | Mouse |
| Detection Range: | 0.312-20ng/ml |
| Sensitivity: | 0.188ng/mL |
| Intra CV: | 3.6% | ||||||||||||||||||||
| Inter CV: | 5.7% | ||||||||||||||||||||
| Linearity: |
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| Recovery: |
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| Function: | Receptor for the Fc region of complexed immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of antigen-antibody complexes from the circulation and modulation of antibody production by B-cells. Isoform IIB1 and isoform IIB1' form caps but fail to mediate endocytosis or phagocytosis. Isoform IIB2 can mediate the endocytosis of soluble immune complexes via clathrin-coated pits. Isoform IIB1 and isoform IIB2 can down-regulate B-cell, T-cell, and mast cell activation when coaggregated to B-cell receptors for AG (BCR), T-cell receptors for AG (TCR), and Fc receptors, respectively. |
| Uniprot: | P08101 |
| Sample Type: | Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids |
| Specificity: | Natural and recombinant mouse Low affinity immunoglobulin gamma Fc region receptor II |
| Sub Unit: | Interacts with FGR (By similarity). Interacts with LYN. |
| Research Area: | Immunology |
| Subcellular Location: | Isoform IIB3 Secreted Released as a soluble molecule. |
| Storage: | Please see kit components below for exact storage details |
| Note: | For research use only |
| UniProt Protein Function: | FCGR2B: a transmembrane receptor for the Fc region of complexed or aggregated immunoglobulin gamma (IgG). Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in down modulation of previous state of cell activation triggered via antigen receptors on B cells, T cells or via another Fc receptor. Three splice-variant isoforms have been observed. |
| UniProt Protein Details: | Protein type:Cell surface; Oncoprotein; Membrane protein, integral Cellular Component: cell surface; cytoplasm; cytoskeleton; external side of plasma membrane; extracellular region; integral to membrane; integral to plasma membrane; lipid raft; membrane; plasma membrane; recycling endosome Molecular Function:IgG binding; immunoglobulin binding; protein binding; receptor signaling protein activity Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; cell surface receptor linked signal transduction; defense response; endocytic recycling; immune complex clearance; immune response; immunoglobulin mediated immune response; mast cell activation; negative regulation of acute inflammatory response to antigenic stimulus; negative regulation of B cell proliferation; negative regulation of humoral immune response mediated by circulating immunoglobulin; negative regulation of immune response; negative regulation of immunoglobulin production; negative regulation of inflammatory response to antigenic stimulus; negative regulation of interleukin-10 production; negative regulation of neutrophil apoptosis; negative regulation of phagocytosis; negative regulation of type I hypersensitivity; phagocytosis, engulfment; positive regulation of phagocytosis; receptor-mediated endocytosis; response to bacterium |
| UniProt Code: | P08101 |
| NCBI GenInfo Identifier: | 2506779 |
| NCBI Gene ID: | 14130 |
| NCBI Accession: | P08101.2 |
| UniProt Secondary Accession: | P08101,P08102, P12316, P97917, Q60938, Q60939, Q60940 Q61170, Q61558, |
| UniProt Related Accession: | P08101 |
| Molecular Weight: | |
| NCBI Full Name: | Low affinity immunoglobulin gamma Fc region receptor II |
| NCBI Synonym Full Names: | Fc receptor, IgG, low affinity IIb |
| NCBI Official Symbol: | Fcgr2b |
| NCBI Official Synonym Symbols: | CD32; Fcgr2; Fcr-2; Fcr-3; Ly-17; LyM-1; Lym-1; fcRII; FcgRII; Fcgr2a; Ly-m20; AI528646; Fc[g]RII; F630109E10Rik |
| NCBI Protein Information: | low affinity immunoglobulin gamma Fc region receptor II-b |
| UniProt Protein Name: | Low affinity immunoglobulin gamma Fc region receptor II |
| UniProt Synonym Protein Names: | Fc gamma receptor IIB; Fc-gamma RII; Fc-gamma-RIIB; FcRII; IgG Fc receptor II beta; Lymphocyte antigen 17; Ly-17; CD_antigen: CD32 |
| UniProt Gene Name: | Fcgr2 |
| UniProt Entry Name: | FCGR2_MOUSE |
| Component | Quantity (96 Assays) | Storage |
| ELISA Microplate (Dismountable) | 8×12 strips | -20°C |
| Lyophilized Standard | 2 | -20°C |
| Sample Diluent | 20ml | -20°C |
| Assay Diluent A | 10mL | -20°C |
| Assay Diluent B | 10mL | -20°C |
| Detection Reagent A | 120µL | -20°C |
| Detection Reagent B | 120µL | -20°C |
| Wash Buffer | 30mL | 4°C |
| Substrate | 10mL | 4°C |
| Stop Solution | 10mL | 4°C |
| Plate Sealer | 5 | - |
Other materials and equipment required:
- Microplate reader with 450 nm wavelength filter
- Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
- Incubator
- Deionized or distilled water
- Absorbent paper
- Buffer resevoir
*Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Allow all reagents to reach room temperature (Please do not dissolve the reagents at 37°C directly). All the reagents should be mixed thoroughly by gently swirling before pipetting. Avoid foaming. Keep appropriate numbers of strips for 1 experiment and remove extra strips from microtiter plate. Removed strips should be resealed and stored at -20°C until the kits expiry date. Prepare all reagents, working standards and samples as directed in the previous sections. Please predict the concentration before assaying. If values for these are not within the range of the standard curve, users must determine the optimal sample dilutions for their experiments. We recommend running all samples in duplicate.
| Step | |
| 1. | Add Sample: Add 100µL of Standard, Blank, or Sample per well. The blank well is added with Sample diluent. Solutions are added to the bottom of micro ELISA plate well, avoid inside wall touching and foaming as possible. Mix it gently. Cover the plate with sealer we provided. Incubate for 120 minutes at 37°C. |
| 2. | Remove the liquid from each well, don't wash. Add 100µL of Detection Reagent A working solution to each well. Cover with the Plate sealer. Gently tap the plate to ensure thorough mixing. Incubate for 1 hour at 37°C. Note: if Detection Reagent A appears cloudy warm to room temperature until solution is uniform. |
| 3. | Aspirate each well and wash, repeating the process three times. Wash by filling each well with Wash Buffer (approximately 400µL) (a squirt bottle, multi-channel pipette,manifold dispenser or automated washer are needed). Complete removal of liquid at each step is essential. After the last wash, completely remove remaining Wash Buffer by aspirating or decanting. Invert the plate and pat it against thick clean absorbent paper. |
| 4. | Add 100µL of Detection Reagent B working solution to each well. Cover with the Plate sealer. Incubate for 60 minutes at 37°C. |
| 5. | Repeat the wash process for five times as conducted in step 3. |
| 6. | Add 90µL of Substrate Solution to each well. Cover with a new Plate sealer and incubate for 10-20 minutes at 37°C. Protect the plate from light. The reaction time can be shortened or extended according to the actual color change, but this should not exceed more than 30 minutes. When apparent gradient appears in standard wells, user should terminatethe reaction. |
| 7. | Add 50µL of Stop Solution to each well. If color change does not appear uniform, gently tap the plate to ensure thorough mixing. |
| 8. | Determine the optical density (OD value) of each well at once, using a micro-plate reader set to 450 nm. User should open the micro-plate reader in advance, preheat the instrument, and set the testing parameters. |
| 9. | After experiment, store all reagents according to the specified storage temperature respectively until their expiry. |
When carrying out an ELISA assay it is important to prepare your samples in order to achieve the best possible results. Below we have a list of procedures for the preparation of samples for different sample types.
| Sample Type | Protocol |
| Serum | If using serum separator tubes, allow samples to clot for 30 minutes at room temperature. Centrifuge for 10 minutes at 1,000x g. Collect the serum fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. If serum separator tubes are not being used, allow samples to clot overnight at 2-8°C. Centrifuge for 10 minutes at 1,000x g. Remove serum and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. |
| Plasma | Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples at 4°C for 15 mins at 1000 × g within 30 mins of collection. Collect the plasma fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. Note: Over haemolysed samples are not suitable for use with this kit. |
| Urine & Cerebrospinal Fluid | Collect the urine (mid-stream) in a sterile container, centrifuge for 20 mins at 2000-3000 rpm. Remove supernatant and assay immediately. If any precipitation is detected, repeat the centrifugation step. A similar protocol can be used for cerebrospinal fluid. |
| Cell culture supernatant | Collect the cell culture media by pipette, followed by centrifugation at 4°C for 20 mins at 1500 rpm. Collect the clear supernatant and assay immediately. |
| Cell lysates | Solubilize cells in lysis buffer and allow to sit on ice for 30 minutes. Centrifuge tubes at 14,000 x g for 5 minutes to remove insoluble material. Aliquot the supernatant into a new tube and discard the remaining whole cell extract. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C. |
| Tissue homogenates | The preparation of tissue homogenates will vary depending upon tissue type. Rinse tissue with 1X PBS to remove excess blood & homogenize in 20ml of 1X PBS (including protease inhibitors) and store overnight at ≤ -20°C. Two freeze-thaw cycles are required to break the cell membranes. To further disrupt the cell membranes you can sonicate the samples. Centrifuge homogenates for 5 mins at 5000xg. Remove the supernatant and assay immediately or aliquot and store at -20°C or -80°C. |
| Tissue lysates | Rinse tissue with PBS, cut into 1-2 mm pieces, and homogenize with a tissue homogenizer in PBS. Add an equal volume of RIPA buffer containing protease inhibitors and lyse tissues at room temperature for 30 minutes with gentle agitation. Centrifuge to remove debris. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C. |
| Breast Milk | Collect milk samples and centrifuge at 10,000 x g for 60 min at 4°C. Aliquot the supernatant and assay. For long term use, store samples at -80°C. Minimize freeze/thaw cycles. |
