The Mouse LOX (lysyl oxidase) CLIA Kit is a reliable and sensitive assay designed for the accurate detection of lysyl oxidase levels in mouse serum, plasma, and cell culture supernatants. This kit offers high specificity and precision, ensuring consistent and trustworthy results for a variety of research applications.Lysyl oxidase is a key enzyme involved in the cross-linking of collagen and elastin in the extracellular matrix, playing a critical role in tissue remodeling and fibrosis. Abnormal lysyl oxidase expression has been implicated in various diseases, including cancer, fibrosis, and cardiovascular conditions, making it a valuable biomarker for investigating these disorders and exploring potential therapeutic interventions.
Overall, the Mouse LOX CLIA Kit provides researchers with a powerful tool for studying lysyl oxidase biology and its role in disease pathology, offering a comprehensive solution for accurate and reliable quantification of this important enzyme in mouse samples.
Product Name:
Mouse LOX (Lysyl Oxidase) CLIA Kit
SKU:
MOES00005
Target:
Mouse LOX (Lysyl Oxidase)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse LOX. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse LOX and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse LOX, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse LOX. You can calculate the concentration of Mouse LOX in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-104
84-96
99-112
Average (%)
95
90
106
1:4
Range (%)
85-98
97-114
94-111
Average (%)
92
105
102
1:8
Range (%)
89-106
90-105
87-98
Average (%)
97
98
93
1:16
Range (%)
99-116
83-95
89-104
Average (%)
106
90
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-98
91
EDTA plasma (n=5)
93-104
99
Cell culture media (n=5)
86-99
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
198.56
563.17
1475.33
207.04
570.4
1387.73
Standard deviation
19.36
64.37
97.08
19.25
57.67
89.23
C V (%)
9.75
11.43
6.58
9.3
10.11
6.43
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse LOX concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse LOX in samples. No significant cross-reactivity or interference between Mouse LOX and analogues was observed.
