The Mouse LPTN (XCL1) Lymphotactin CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of LPTN (XCL1) levels in mouse serum, plasma, and cell culture supernatants. This kit offers reliable and reproducible results, making it ideal for a variety of research applications.LPTN (XCL1), also known as lymphotactin, is a chemokine that plays a crucial role in the immune response by attracting lymphocytes to sites of inflammation.
It is involved in various immune-related processes, making it a valuable biomarker for studying immune system function and inflammatory conditions.By utilizing the Mouse LPTN (XCL1) Lymphotactin CLIA Kit, researchers can gain valuable insights into the role of LPTN (XCL1) in immune responses and inflammation, leading to a better understanding of these processes and potentially opening new avenues for therapeutic interventions.
Product Name:
Mouse Lptn/XCL1 (Lymphotactin) CLIA Kit
SKU:
MOES00428
Target:
Mouse Lptn/XCL1 (Lymphotactin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse Lptn/XCL1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse Lptn/XCL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse Lptn/XCL1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse Lptn/XCL1. You can calculate the concentration of Mouse Lptn/XCL1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-108
89-101
92-105
Average (%)
101
94
97
1:4
Range (%)
86-102
97-113
98-114
Average (%)
93
103
104
1:8
Range (%)
100-116
93-105
102-115
Average (%)
106
100
109
1:16
Range (%)
97-110
92-104
98-115
Average (%)
103
97
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-103
95
EDTA plasma (n=5)
88-103
95
Cell culture media (n=5)
89-102
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
50.45
137.81
354.18
47.46
129.87
348.52
Standard deviation
5.4
11.16
27.84
4.47
11.87
26.77
C V (%)
10.7
8.1
7.86
9.42
9.14
7.68
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse Lptn/XCL1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse Lptn/XCL1 in samples. No significant cross-reactivity or interference between Mouse Lptn/XCL1 and analogues was observed.
