The Mouse M-CSF (Macrophage Colony-Stimulating Factor) CLIA Kit is a reliable and accurate tool for detecting M-CSF levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for your research needs.M-CSF is a key cytokine that plays a critical role in the regulation of macrophage and monocyte development and function. It is involved in various physiological processes, including immune response, tissue repair, and bone metabolism.
Dysregulation of M-CSF levels is associated with inflammatory diseases, autoimmune disorders, and cancer, highlighting its importance as a biomarker for studying these conditions.With its advanced technology and superior performance, the Mouse M-CSF CLIA Kit is an essential tool for investigating the role of M-CSF in disease pathogenesis and identifying potential therapeutic targets. Trust in this kit to provide you with accurate and reliable data for your research projects.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse M-CSF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse M-CSF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse M-CSF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse M-CSF. You can calculate the concentration of Mouse M-CSF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-113
88-101
97-107
Average (%)
105
95
102
1:4
Range (%)
87-101
101-121
89-102
Average (%)
93
110
95
1:8
Range (%)
95-110
101-116
90-107
Average (%)
102
110
98
1:16
Range (%)
84-98
86-99
98-111
Average (%)
91
92
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-104
95
EDTA plasma (n=5)
94-105
100
Cell culture media (n=5)
90-105
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.69
79.38
233.84
24.21
73.21
223.76
Standard deviation
3.14
8.04
16.84
2.31
5.59
16.49
C V (%)
12.22
10.13
7.2
9.54
7.64
7.37
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse M-CSF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse M-CSF in samples. No significant cross-reactivity or interference between Mouse M-CSF and analogues was observed.
