The Mouse Matrix Metalloproteinase-9 (MMP9) ELISA Kit is specially designed for the precise measurement of MMP9 levels in mouse serum, plasma, and tissue lysates. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results, making it perfect for a variety of research purposes.MMP9 is a key enzyme involved in extracellular matrix remodeling, cell migration, and tissue repair. Dysregulation of MMP9 has been linked to various pathologies such as cancer, inflammatory diseases, and cardiovascular disorders, highlighting its importance as a potential therapeutic target and biomarker.
With the Mouse MMP9 ELISA Kit, researchers can effectively study the role of MMP9 in disease progression, assess treatment efficacy, and discover novel therapeutic interventions. Invest in this reliable and high-quality kit to advance your research in the field of matrix metalloproteinases and their impact on health and disease.
Product Name:
Mouse Matrix Metalloproteinase 9 (MMP9) ELISA Kit
Product Code:
MODL00882
Size:
96 Assays
Target:
MMP9
Synonyms:
GELB, Gelatinase B, CLG4B, CLG4-B, 92 KDa Gelatinase, 92kDa Type IV Collagenase
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
