Mouse MCP-3 (Monocyte Chemotactic Protein 3) CLIA Kit
The Mouse MCP-3 (Monocyte Chemotactic Protein-3) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of MCP-3 levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.MCP-3 is a key chemokine involved in immune responses and inflammation, playing a crucial role in recruiting monocytes and other immune cells to sites of injury or infection. Dysregulation of MCP-3 levels has been implicated in various inflammatory conditions, making it a valuable biomarker for studying immune responses and developing new therapeutic interventions.
With its high sensitivity and specificity, the Mouse MCP-3 CLIA Kit is a valuable tool for researchers looking to investigate the role of MCP-3 in immune function, inflammation, and disease. Its ease of use and accuracy make it a reliable choice for laboratories conducting preclinical and clinical research studies.
Product Name:
Mouse MCP-3 (Monocyte Chemotactic Protein 3) CLIA Kit
SKU:
MOES00440
Target:
Mouse MCP-3 (Monocyte Chemotactic Protein 3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse MCP-3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MCP-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MCP-3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse MCP-3. You can calculate the concentration of Mouse MCP-3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-114
95-108
97-115
Average (%)
104
102
105
1:4
Range (%)
95-109
87-98
87-100
Average (%)
102
93
93
1:8
Range (%)
86-100
90-104
89-100
Average (%)
93
95
95
1:16
Range (%)
87-100
98-113
97-111
Average (%)
94
106
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-99
94
EDTA plasma (n=5)
92-104
98
Cell culture media (n=5)
99-115
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
27.19
78.09
181.17
28.72
73.16
170.54
Standard deviation
2.34
8.4
15.06
2.45
8.52
14.33
C V (%)
8.61
10.76
8.31
8.53
11.65
8.4
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse MCP-3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse MCP-3 in samples. No significant cross-reactivity or interference between Mouse MCP-3 and analogues was observed.
