Mouse Monocyte Chemotactic Protein 3 (MCP3) ELISA Kit
The Mouse Monocyte Chemotactic Protein 3 (MCP3) ELISA Kit is a powerful tool for researchers looking to accurately detect MCP3 levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit offers reliable and reproducible results, making it suitable for a wide range of research applications.MCP3 is a key chemokine involved in recruiting monocytes to sites of inflammation, playing a crucial role in the immune response.
Its dysregulation has been implicated in various inflammatory diseases, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.Overall, the Mouse MCP3 ELISA Kit provides researchers with a valuable tool for studying the role of MCP3 in inflammation and immune response, offering precise and accurate measurements to advance their research goals.
Product Name:
Mouse Monocyte Chemotactic Protein 3 (MCP3) ELISA Kit
Product Code:
MODL00850
Size:
96 Assays
Target:
MCP3
Synonyms:
CCL7, FIC, MARC, NC28, MCP3, SCYA7, Chemokine C-C-Motif Ligand 7, Small Inducible Cytokine A7, Monocyte Chemoattractant Protein 3
Detection Method:
Sandwich
Range:
15.625-1,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
