The Mouse MDC (CCL22) ELISA Kit is specifically designed for the precise detection of macrophage-derived chemokine levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.Macrophage-derived chemokine, also known as CCL22, plays a critical role in immune responses and inflammatory processes.
It is involved in recruiting immune cells to sites of inflammation and regulating immune cell migration. The Mouse MDC ELISA Kit is essential for studying immune function, inflammation, and potential therapeutic interventions in various disease states.Order your Mouse MDC (CCL22) ELISA Kit today from Assay Genie and unlock new insights into immune response mechanisms and inflammatory pathways in mouse models.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse MDC/CCL22. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MDC/CCL22 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MDC/CCL22, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse MDC/CCL22. You can calculate the concentration of Mouse MDC/CCL22 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-102
95-110
89-104
Average (%)
97
101
95
1:4
Range (%)
90-104
86-98
89-100
Average (%)
97
91
94
1:8
Range (%)
90-104
87-101
86-97
Average (%)
97
92
91
1:16
Range (%)
91-106
84-99
87-99
Average (%)
99
90
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-110
101
EDTA plasma (n=5)
85-97
91
Cell culture media (n=5)
85-96
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
46.55
151.02
428.84
44.54
138.94
427.16
Standard deviation
2.91
6.6
18.44
2.72
8.27
14.91
C V (%)
6.25
4.37
4.3
6.11
5.95
3.49
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse MDC/CCL22 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse MDC/CCL22 in samples. No significant cross-reactivity or interference between Mouse MDC/CCL22 and analogues was observed.
