PerCP/Cyanine5.5 Anti-Mouse MHC II (I-A/I-E) Antibody [M5/114]
The Mouse MHC II (I-A/I-E) Monoclonal Antibody (PerCP-Cyanine5.5 Conjugated) M5/114 (AGEL0497) is a specialized antibody tailored for the detection of major histocompatibility complex class II (MHC II) molecules in mouse samples. MHC II molecules play a vital role in the immune system by presenting antigens to T-cells, triggering immune responses against pathogens.This monoclonal antibody, conjugated with PerCP-Cyanine5.5 for fluorescent visualization, specifically targets the I-A and I-E subtypes of MHC II in mouse cells. It is ideal for flow cytometry applications, allowing for precise and accurate analysis of MHC II expression on immune cells in various experimental settings.
The M5/114 antibody provides researchers with a powerful tool to investigate antigen presentation, T-cell activation, and immune response modulation in mouse models. By enabling the visualization and quantification of MHC II molecules, this antibody facilitates studies in immunology, infectious diseases, vaccine development, and autoimmune conditions, contributing valuable insights to the field of biomedical research.
Product Title:
PerCP/Cyanine5.5 Anti-Mouse MHC II (I-A/I-E) Antibody [M5/114]
SKU:
AGEL0497
Size:
50 Tests, 100 Tests, 200 Tests
Clone No:
M5/114
Synonyms:
H2-Ab1/Eb1;Major histocompatibility protein class II beta chain;MHC class II H2-IA-beta-psi;I-E beta MHC class II;MHC class II;
Host Species:
Rat
Reactivity:
Mouse
Application:
FCM
Isotype:
Rat IgG2b, κ
Isotype Control:
PerCP/Cyanine5.5 Rat IgG2b, κ Isotype Control[LTF-2]
Form:
Liquid
Conjugation:
PerCP/Cyanine 5.5
Recommended Use:
Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Spectrum:
Swissprot:
P14483 O78196
Gene ID:
14961,14969
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b,d,q,r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins.
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