PerCP/Cyanine5.5 Anti-Mouse MHC II (I-A/I-E) Antibody [M5/114]
The Mouse MHC II (I-A/I-E) Monoclonal Antibody (PerCP-Cyanine5.5 Conjugated) M5/114 (AGEL0506) is an essential tool for researchers studying immune responses and antigen presentation in mice. This monoclonal antibody specifically targets the major histocompatibility complex class II (MHC II) proteins I-A and I-E, key players in the activation of T cells and the initiation of adaptive immune responses.By utilizing this antibody, researchers can accurately detect and analyze MHC II expression in various cell types, providing crucial insights into the mechanisms of antigen presentation and immune regulation in mouse models. The PerCP-Cyanine5.5 conjugation enhances the sensitivity and specificity of detection, allowing for precise quantification and localization of MHC II molecules in experimental settings.The M5/114 antibody is well-suited for a wide range of applications, including flow cytometry, immunofluorescence microscopy, and immunohistochemistry, enabling versatile research approaches in immunology, infectious diseases, and vaccine development.
Its reliable performance and consistent results make it a valuable tool for characterizing immune responses and investigating the role of MHC II molecules in host defense and autoimmune disorders.Whether studying immune system function, evaluating vaccine responses, or exploring immune-mediated pathologies, the Mouse MHC II (I-A/I-E) Monoclonal Antibody (PerCP-Cyanine5.5 Conjugated) M5/114 (AGEL0506) is an indispensable reagent for researchers seeking to deepen their understanding of the complex interplay between antigens and the adaptive immune system in mouse models.
Product Title:
PerCP/Cyanine5.5 Anti-Mouse MHC II (I-A/I-E) Antibody [M5/114]
SKU:
AGEL0506
Size:
25µg, 100µg
Clone No:
M5/114
Synonyms:
H2-Ab1/Eb1;Major histocompatibility protein class II beta chain;MHC class II H2-IA-beta-psi;I-E beta MHC class II;MHC class II;
Host Species:
Rat
Reactivity:
Mouse
Application:
FCM
Isotype:
Rat IgG2b, κ
Isotype Control:
PerCP/Cyanine5.5 Rat IgG2b, κ Isotype Control[LTF-2]
Form:
Liquid
Conjugation:
PerCP/Cyanine 5.5
Recommended Use:
Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume].
Spectrum:
Swissprot:
P14483 O78196
Gene ID:
14961,14969
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b,d,q,r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins.
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