Mouse Microsomal Glutathione S Transferase 1 (MGST1) ELISA Kit
The Mouse Microsomal Glutathione S-Transferase 1 (MGST1) ELISA Kit is a reliable and accurate tool for detecting MGST1 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.MGST1 is an important enzyme involved in the detoxification of xenobiotics and oxidative stress. Its role in maintaining cellular homeostasis makes it a key player in various physiological processes and disease pathways.
Studying MGST1 levels can provide valuable insights into conditions such as inflammation, cancer, and neurodegenerative disorders, making this ELISA kit a valuable asset for researchers in these fields.With its easy-to-use format and robust performance, the Mouse MGST1 ELISA Kit is a powerful tool for investigating the role of MGST1 in health and disease, leading to potential therapeutic advancements in the future.
Product Name:
Mouse Microsomal Glutathione S Transferase 1 (MGST1) ELISA Kit
Product Code:
MODL00861
Size:
96 Assays
Target:
MGST1
Synonyms:
GST12 MGST-I
Detection Method:
Sandwich
Range:
1.56-100U/L
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
