Mouse MIP-1 beta (Macrophage Inflammatory Protein 1 Beta) CLIA Kit
The Mouse MIP-1 beta (Macrophage Inflammatory Protein-1 beta) CLIA Kit is a highly sensitive and specific assay designed for the accurate quantification of MIP-1 beta levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an ideal tool for a wide range of research applications.MIP-1 beta is a key chemokine involved in inflammatory responses, attracting immune cells to sites of inflammation and infection. It plays a critical role in regulating the immune system and modulating the inflammatory process.
Elevated levels of MIP-1 beta have been linked to various inflammatory conditions, making it a valuable biomarker for studying immune responses and developing potential therapies.With its high sensitivity and specificity, the Mouse MIP-1 beta CLIA Kit is a valuable tool for researchers interested in studying immune responses, inflammation, and the role of chemokines in disease pathogenesis. Trust Assay Genie for accurate and reliable results in your research studies.
Product Name:
Mouse MIP-1 beta (Macrophage Inflammatory Protein 1 Beta) CLIA Kit
SKU:
MOES00008
Target:
Mouse MIP-1 beta (Macrophage Inflammatory Protein 1 Beta)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse MIP-1β. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MIP-1β and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MIP-1β, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse MIP-1β. You can calculate the concentration of Mouse MIP-1β in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-99
89-104
85-98
Average (%)
93
97
90
1:4
Range (%)
97-113
83-97
90-105
Average (%)
103
90
98
1:8
Range (%)
96-108
95-113
102-115
Average (%)
102
103
109
1:16
Range (%)
97-109
101-118
100-113
Average (%)
103
108
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-109
101
EDTA plasma (n=5)
94-110
101
Cell culture media (n=5)
94-111
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.78
56.3
210.5
26.95
53.13
200.92
Standard deviation
2.15
6.59
13.83
3.32
4.73
16.9
C V (%)
8.68
11.71
6.57
12.32
8.9
8.41
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse MIP-1β concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse MIP-1β in samples. No significant cross-reactivity or interference between Mouse MIP-1β and analogues was observed.
