The Mouse MMP-1 (Matrix Metalloproteinase 1) CLIA Kit is a powerful tool for accurately measuring MMP-1 levels in mouse tissue samples, serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers reliable and consistent results, making it ideal for a variety of research applications.MMP-1 is a key enzyme involved in extracellular matrix remodeling, playing a crucial role in tissue repair, inflammation, and cancer progression. By accurately measuring MMP-1 levels, researchers can gain valuable insights into various biological processes and disease states, ultimately leading to the identification of potential therapeutic targets.
Whether studying cancer metastasis, arthritis development, or tissue remodeling, the Mouse MMP-1 CLIA Kit provides researchers with a reliable and accurate method for quantifying MMP-1 levels, making it an essential tool for advancing scientific knowledge and uncovering new treatment strategies.
Product Name:
Mouse MMP-1 (Matrix Metalloproteinase 1) CLIA Kit
SKU:
MOES00433
Target:
Mouse MMP-1 (Matrix Metalloproteinase 1)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse MMP-1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MMP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MMP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse MMP-1. You can calculate the concentration of Mouse MMP-1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-98
95-109
93-109
Average (%)
92
101
101
1:4
Range (%)
94-112
99-112
103-121
Average (%)
102
105
110
1:8
Range (%)
96-112
96-109
84-99
Average (%)
103
104
91
1:16
Range (%)
93-108
93-108
99-115
Average (%)
100
99
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-103
95
EDTA plasma (n=5)
96-110
102
Cell culture media (n=5)
97-113
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
98.43
238.02
700.89
89.5
229.28
640.83
Standard deviation
10.89
19.73
61.96
8.76
19.31
65.49
C V (%)
11.06
8.29
8.84
9.79
8.42
10.22
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse MMP-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse MMP-1 in samples. No significant cross-reactivity or interference between Mouse MMP-1 and analogues was observed.
