The Mouse MMP-17 (Matrix Metalloproteinase 17) CLIA Kit is specifically designed for the quantitative determination of MMP-17 levels in mouse serum, plasma, and cell culture supernatants. This kit provides high sensitivity and specificity, ensuring accurate and reliable results that can be used for a variety of research applications.MMP-17, also known as membrane-type matrix metalloproteinase 4 (MT4-MMP), plays a key role in the breakdown of extracellular matrix proteins and is involved in processes such as tumor invasion, angiogenesis, and tissue remodeling.
Dysregulation of MMP-17 has been linked to various diseases, including cancer and inflammatory disorders, making it a valuable biomarker for studying these conditions and developing targeted therapies.With its advanced technology and user-friendly format, the Mouse MMP-17 CLIA Kit is an essential tool for researchers looking to investigate the role of MMP-17 in physiological and pathological processes, providing valuable insights for potential diagnostic and therapeutic applications.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse MMP-17. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MMP-17 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MMP-17, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse MMP-17. You can calculate the concentration of Mouse MMP-17 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-117
102-117
101-117
Average (%)
107
109
108
1:4
Range (%)
99-114
92-108
90-101
Average (%)
105
99
96
1:8
Range (%)
99-115
90-105
85-100
Average (%)
106
97
92
1:16
Range (%)
96-113
99-117
99-111
Average (%)
103
107
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-116
107
EDTA plasma (n=5)
84-98
91
Cell culture media (n=5)
87-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
104.31
232.11
886.2
100.15
242.45
831.58
Standard deviation
10.31
21.8
68.33
11.17
18.28
69.19
C V (%)
9.88
9.39
7.71
11.15
7.54
8.32
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse MMP-17 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse MMP-17 in samples. No significant cross-reactivity or interference between Mouse MMP-17 and analogues was observed.
