The Mouse MMP-24 (Matrix Metalloproteinase 24) CLIA Kit is a high-quality assay kit specifically designed for the accurate detection of MMP-24 levels in mouse biological samples. This kit offers high sensitivity and specificity, ensuring precise and reliable results for various research applications.MMP-24 is a key enzyme involved in extracellular matrix remodeling and is known to play a critical role in various physiological and pathological processes, including tissue development, inflammation, and cancer progression.
By accurately measuring MMP-24 levels, researchers can gain valuable insights into disease mechanisms and potential therapeutic targets.Overall, the Mouse MMP-24 CLIA Kit is a valuable tool for researchers studying the role of MMP-24 in different biological processes, providing a reliable platform for further exploration and discovery.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse MMP-24. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MMP-24 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MMP-24, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse MMP-24. You can calculate the concentration of Mouse MMP-24 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-114
100-115
89-102
Average (%)
106
107
95
1:4
Range (%)
89-103
88-102
97-115
Average (%)
96
94
105
1:8
Range (%)
103-117
101-116
94-107
Average (%)
109
107
101
1:16
Range (%)
91-103
90-107
102-116
Average (%)
97
98
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-106
100
EDTA plasma (n=5)
98-112
105
Cell culture media (n=5)
90-104
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
94.81
254.73
717.95
88.42
258.29
782.09
Standard deviation
8.18
27.84
45.3
7.18
25.21
55.84
C V (%)
8.63
10.93
6.31
8.12
9.76
7.14
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse MMP-24 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse MMP-24 in samples. No significant cross-reactivity or interference between Mouse MMP-24 and analogues was observed.
