The Mouse MMP-7 (Matrix Metalloproteinase-7) CLIA Kit is specifically designed for the quantitative detection of MMP-7 levels in mouse serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, ensuring accurate and reliable results for research applications.MMP-7 is a key enzyme involved in extracellular matrix degradation and tissue remodeling. It plays a crucial role in various physiological and pathological processes, including wound healing, tissue repair, inflammation, and cancer progression.
Monitoring MMP-7 levels can provide valuable insights into disease mechanisms and potential therapeutic targets.With its easy-to-use format and reliable performance, the Mouse MMP-7 CLIA Kit is an essential tool for researchers studying the role of MMP-7 in various biological processes and disease states. Get accurate and reproducible results with this innovative kit from AssayGenie.
Product Name:
Mouse MMP-7 (Matrix Metalloproteinase 7) CLIA Kit
SKU:
MOES00437
Target:
Mouse MMP-7 (Matrix Metalloproteinase 7)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse MMP-7. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MMP-7 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MMP-7, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse MMP-7. You can calculate the concentration of Mouse MMP-7 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-100
97-112
91-102
Average (%)
94
103
97
1:4
Range (%)
90-103
102-115
92-104
Average (%)
97
108
98
1:8
Range (%)
101-120
92-107
99-112
Average (%)
109
98
105
1:16
Range (%)
100-115
95-110
94-110
Average (%)
108
102
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-99
93
EDTA plasma (n=5)
86-99
93
Cell culture media (n=5)
94-110
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
47.02
123.69
416.57
46.55
133.75
427.38
Standard deviation
6.03
8.96
37.82
4.23
11.81
46.03
C V (%)
12.82
7.24
9.08
9.09
8.83
10.77
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse MMP-7 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse MMP-7 in samples. No significant cross-reactivity or interference between Mouse MMP-7 and analogues was observed.
