The Mouse Myosin Va (MYO5A) ELISA Kit is specifically designed for the quantitative detection of MYO5A levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for various research applications.MYO5A is a key motor protein that plays a vital role in intracellular transport and organelle positioning. Dysregulation of MYO5A has been linked to various neurological disorders, including Alzheimer's disease and Parkinson's disease.
By measuring MYO5A levels, researchers can gain valuable insights into disease mechanisms and identify potential therapeutic targets.Overall, the Mouse Myosin Va (MYO5A) ELISA Kit is a valuable tool for studying the role of MYO5A in physiological and pathological processes, offering researchers a reliable and efficient method for analyzing MYO5A levels in mouse samples.
Product Name:
Mouse Myosin VA (MYO5A) ELISA Kit
Product Code:
MODL00915
Size:
96 Assays
Target:
MYO5A
Synonyms:
GS1, MYH12, MYO5, MYOXIN, MYR12, myosin V, myosin Va, Myosin,Heavy Polypeptide Kinase, Myosin Heavy Chain 12, Myoxin, Myosin V, Dilute myosin heavy chain, non-muscle
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-99
94
Heparin plasma (n=5)
91-101
96
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
