The Mouse NFIL3 CLIA Kit is a cutting-edge assay designed for the quantitative measurement of NFIL3 levels in mouse serum, plasma, and tissue lysate samples. This kit boasts high sensitivity and specificity, guaranteeing accurate and consistent results for a variety of research applications.NFIL3, also known as E4BP4, is a transcription factor that plays a critical role in regulating immune responses, circadian rhythms, and cell differentiation. Dysregulation of NFIL3 has been linked to various diseases, including autoimmune disorders, cancer, and inflammatory conditions, making it a valuable target for further investigation and potential therapeutic interventions.
With its user-friendly protocol and reliable performance, the Mouse NFIL3 CLIA Kit is a valuable tool for researchers studying NFIL3 biology and its impact on health and disease. Advance your research with confidence using this innovative assay.
Product Name:
Mouse NFIL-3 CLIA Kit
SKU:
MOES00456
Target:
Mouse NFIL-3
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse NFIL3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse NFIL3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse NFIL3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse NFIL3. You can calculate the concentration of Mouse NFIL3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-101
99-114
84-97
Average (%)
95
106
91
1:4
Range (%)
96-111
99-117
95-109
Average (%)
104
107
102
1:8
Range (%)
86-101
94-108
98-113
Average (%)
93
101
105
1:16
Range (%)
86-100
91-107
100-115
Average (%)
93
99
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-103
95
EDTA plasma (n=5)
89-99
94
Cell culture media (n=5)
101-113
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
205.45
349.61
1769.81
207.84
373.62
1824.31
Standard deviation
24.22
30.87
146.89
22.16
27.76
147.59
C V (%)
11.79
8.83
8.3
10.66
7.43
8.09
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse NFIL3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse NFIL3 in samples. No significant cross-reactivity or interference between Mouse NFIL3 and analogues was observed.
