The Mouse NGAL (Neutrophil Gelatinase-Associated Lipocalin) CLIA Kit is a powerful tool for accurate measurement of NGAL levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, delivering precise and consistent results for a variety of research purposes.NGAL is a key biomarker associated with kidney injury and inflammation, providing valuable insights into renal function and disease progression. By quantifying NGAL levels, researchers can better understand the mechanisms underlying kidney disorders and explore potential therapeutic interventions.
With its reliable performance and versatility, the Mouse NGAL CLIA Kit is an indispensable resource for investigating kidney-related conditions and advancing the development of targeted treatments. Trust in this kit to enhance your research efforts and contribute to advancements in the field of kidney health.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse NGAL. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse NGAL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse NGAL, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse NGAL. You can calculate the concentration of Mouse NGAL in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-115
96-113
85-98
Average (%)
109
104
91
1:4
Range (%)
86-100
104-119
86-98
Average (%)
92
110
93
1:8
Range (%)
103-117
94-109
86-100
Average (%)
109
100
92
1:16
Range (%)
89-104
94-107
86-96
Average (%)
96
100
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-106
98
EDTA plasma (n=5)
100-114
106
Cell culture media (n=5)
91-104
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
47.93
122.0
435.64
44.28
125.61
472.25
Standard deviation
5.39
11.99
37.33
4.68
14.99
49.35
C V (%)
11.25
9.83
8.57
10.57
11.93
10.45
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse NGAL concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse NGAL in samples. No significant cross-reactivity or interference between Mouse NGAL and analogues was observed.
