The Mouse NGF (Nerve Growth Factor) CLIA Kit is specifically designed for the quantitative detection of NGF levels in mouse serum, plasma, and cell culture supernatants. This advanced kit offers high sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications.NGF is a key protein that plays a vital role in promoting the growth, survival, and function of neurons. It is crucial for proper nervous system development and function, making it a valuable biomarker for studying neurodegenerative disorders, nerve regeneration, and other neurological conditions.
By utilizing the Mouse NGF CLIA Kit, researchers can gain valuable insights into the role of NGF in various physiological and pathological processes, ultimately leading to a better understanding of neurological disorders and the development of potential therapeutic interventions. This kit is an essential tool for any laboratory conducting studies related to nerve growth factor and its impact on nervous system health.
Product Name:
Mouse NGF (Nerve growth factor) CLIA Kit
SKU:
MOES00446
Target:
Mouse NGF (Nerve growth factor)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse NGF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse NGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse NGF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse NGF. You can calculate the concentration of Mouse NGF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
103-115
88-102
99-114
Average (%)
109
93
105
1:4
Range (%)
87-100
98-115
84-96
Average (%)
93
106
91
1:8
Range (%)
98-113
85-100
93-109
Average (%)
104
92
100
1:16
Range (%)
99-114
102-118
90-104
Average (%)
107
109
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-105
98
EDTA plasma (n=5)
87-100
94
Cell culture media (n=5)
92-104
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
40.05
96.94
395.79
41.08
88.94
369.96
Standard deviation
4.56
9.8
29.25
4.73
7.45
34.37
C V (%)
11.39
10.11
7.39
11.51
8.38
9.29
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse NGF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse NGF in samples. No significant cross-reactivity or interference between Mouse NGF and analogues was observed.
