The Mouse NT-4 (Neurotrophin-4) CLIA Kit is a highly sensitive assay designed for the accurate detection of NT-4 levels in mouse serum, plasma, and tissue culture supernatants. This kit offers high specificity, ensuring reliable and reproducible results for a variety of research applications.Neurotrophin-4 is a critical protein involved in promoting neuronal survival and growth, making it essential for neurodevelopment and maintenance.
Imbalances in NT-4 levels have been implicated in various neurological disorders, making this kit a valuable tool for studying these conditions and potential therapeutic interventions.Overall, the Mouse NT-4 CLIA Kit from AssayGenie provides researchers with a reliable and efficient method for quantifying NT-4 levels, aiding in the advancement of neuroscience research and the development of new treatment strategies.
Product Name:
Mouse NT-4 (Neurotrophin 4) CLIA Kit
SKU:
MOES00451
Target:
Mouse NT-4 (Neurotrophin 4)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse NT-4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse NT-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse NT-4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse NT-4. You can calculate the concentration of Mouse NT-4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-113
86-98
86-99
Average (%)
107
92
93
1:4
Range (%)
103-118
103-116
94-108
Average (%)
110
109
100
1:8
Range (%)
86-99
91-106
95-108
Average (%)
92
98
101
1:16
Range (%)
94-106
100-114
96-112
Average (%)
100
107
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-103
96
EDTA plasma (n=5)
93-108
100
Cell culture media (n=5)
90-103
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.55
48.16
170.96
25.11
47.61
175.32
Standard deviation
2.69
3.67
11.97
2.94
4.41
18.01
C V (%)
11.42
7.62
7.0
11.71
9.26
10.27
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse NT-4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse NT-4 in samples. No significant cross-reactivity or interference between Mouse NT-4 and analogues was observed.
