Mouse NTX I (Cross Linked N-Telopeptide of Type I Collagen) ELISA Kit
The Mouse NTXⅠ (Cross Linked N-Telopeptide of Type Ⅰ Collagen) ELISA Kit is specifically designed to quantitatively measure the levels of NTXⅠ in mouse biological samples. NTXⅠ is a crucial biomarker associated with bone metabolism and resorption processes, providing valuable insights into bone turnover and collagen degradation. Our ELISA kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results for researchers exploring bone health, osteoporosis, and related conditions.
With stringent quality control measures, this kit delivers reliable performance, making it an ideal tool for studying the dynamics of bone remodeling and understanding the pathological mechanisms underlying bone disorders.
Product Name:
Mouse NTX I (Cross Linked N-Telopeptide of Type I Collagen) ELISA Kit
SKU:
AEES00217
Target:
Mouse NTXⅠ (Cross Linked N-Telopeptide of Type Ⅰ Collagen)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse NTXⅠ. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse NTXⅠ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse NTXⅠ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse NTXⅠ. You can calculate the concentration of Mouse NTXⅠ in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-98
91-103
97-107
Average (%)
95
97
100
1:4
Range (%)
92-101
98-111
101-109
Average (%)
95
104
104
1:8
Range (%)
89-102
93-106
100-109
Average (%)
94
101
103
1:16
Range (%)
86-100
89-100
101-109
Average (%)
93
93
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-93
90
EDTA plasma (n=5)
85-97
90
Cell culture media (n=5)
99-113
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.44
1.09
4.79
0.49
1.11
4.82
Standard deviation
0.02
0.05
0.2
0.03
0.05
0.26
C V (%)
4.55
4.59
4.18
6.12
4.5
5.39
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse NTXⅠ concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse NTXⅠ in samples. No significant cross-reactivity or interference between Mouse NTXⅠ and analogues was observed.
