Based on the information provided in the URL www.assaygenie.com/mouse-osm-oncostatin-m-clia-kit-moes00461/, we introduce the Mouse OSM (Oncostatin M) CLIA Kit (MOES00461). This kit is specifically designed for the accurate and reliable quantification of Oncostatin M levels in mouse serum, plasma, and cell culture supernatants.Oncostatin M is a cytokine belonging to the interleukin-6 family, known for its diverse biological functions including regulation of inflammation, cell growth, and differentiation. Dysregulation of Oncostatin M has been linked to various diseases such as cancer, inflammatory disorders, and autoimmune diseases, making it a valuable biomarker for research and potential therapeutic development.
Our Mouse OSM CLIA Kit offers high sensitivity and specificity, ensuring precise and reproducible results for your research needs. With easy-to-follow instructions and optimized assay conditions, this kit is suitable for a wide range of applications in the field of immunology, oncology, and inflammation research. Unlock the potential of your research and discover new insights into the role of Oncostatin M with our high-quality CLIA Kit.
Product Name:
Mouse OSM (Oncostatin-M) CLIA Kit
SKU:
MOES00461
Target:
Mouse OSM (Oncostatin-M)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse OSM. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse OSM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse OSM, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse OSM. You can calculate the concentration of Mouse OSM in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-110
92-106
96-113
Average (%)
103
100
103
1:4
Range (%)
103-115
95-108
103-116
Average (%)
109
102
109
1:8
Range (%)
99-115
93-109
101-113
Average (%)
107
100
107
1:16
Range (%)
86-96
88-101
94-107
Average (%)
91
94
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-111
105
EDTA plasma (n=5)
98-111
105
Cell culture media (n=5)
88-103
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.91
61.15
241.21
23.32
63.34
251.54
Standard deviation
3.05
5.52
17.97
2.78
6.82
17.56
C V (%)
12.24
9.03
7.45
11.92
10.77
6.98
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse OSM concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse OSM in samples. No significant cross-reactivity or interference between Mouse OSM and analogues was observed.
