The Mouse Orexin (OX) ELISA Kit is specifically designed for the precise measurement of orexin levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing trustworthy and consistent results, making it perfect for a variety of research purposes.Orexin is a vital neuropeptide linked to the regulation of various physiological processes, including sleep-wake cycles, appetite, and energy balance. Dysfunction in orexin signaling has been associated with conditions such as narcolepsy, obesity, and mood disorders, making it a valuable biomarker for investigating these disorders and exploring potential therapeutic interventions.
With easy-to-follow instructions and a user-friendly format, the Mouse Orexin (OX) ELISA Kit is an indispensable tool for researchers studying the role of orexin in various physiological and pathological processes in mouse models. Visit our website for more information and to order your kit today.
Product Name:
Mouse Orexin (OX) ELISA Kit
Product Code:
MODL00994
Size:
96 Assays
Target:
OX
Synonyms:
HCRT, PPOX, PPORX, Hypocretin
Detection Method:
Sandwich
Range:
7.812-500pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
