The Mouse PAI-1 (Plasminogen Activator Inhibitor-1) CLIA Kit is a highly sensitive and specific assay designed for the quantification of PAI-1 levels in mouse serum, plasma, and tissue culture samples. This kit provides accurate and reproducible results, making it an essential tool for researchers studying the role of PAI-1 in various physiological and pathological processes.PAI-1 is a key regulator of the fibrinolytic system, inhibiting the activity of plasminogen activators and playing a crucial role in the regulation of blood clot formation and dissolution.
Dysregulation of PAI-1 has been implicated in conditions such as thrombosis, cardiovascular diseases, and inflammation, making it a valuable biomarker for studying these disorders and evaluating potential therapeutic interventions.Overall, the Mouse PAI-1 CLIA Kit offers researchers a reliable and efficient method for measuring PAI-1 levels, providing valuable insights into the mechanisms underlying PAI-1-mediated pathologies and facilitating the development of novel treatment strategies.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse PAI-1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PAI-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PAI-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse PAI-1. You can calculate the concentration of Mouse PAI-1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-118
100-116
91-103
Average (%)
108
108
96
1:4
Range (%)
90-105
97-110
84-97
Average (%)
97
103
90
1:8
Range (%)
97-110
97-110
101-114
Average (%)
104
102
107
1:16
Range (%)
85-95
103-119
93-107
Average (%)
90
109
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-103
96
EDTA plasma (n=5)
90-104
97
Cell culture media (n=5)
99-115
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
94.47
282.0
736.5
100.76
279.41
741.25
Standard deviation
10.39
32.77
77.26
11.69
24.53
76.72
C V (%)
11.0
11.62
10.49
11.6
8.78
10.35
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse PAI-1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse PAI-1 in samples. No significant cross-reactivity or interference between Mouse PAI-1 and analogues was observed.
