The Mouse Pancreatic Polypeptide (PP) ELISA Kit is specifically designed for the detection of PP levels in mouse serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit provides accurate and reproducible results, making it perfect for various research applications.Pancreatic polypeptide (PP) is an important hormone produced by the pancreas that plays a role in regulating food intake, metabolism, and energy balance. Abnormal levels of PP have been associated with conditions such as obesity, diabetes, and pancreatic disorders, making it a valuable biomarker for studying these diseases and potential therapies.
This ELISA kit is easy to use and provides fast results, allowing researchers to efficiently measure PP levels in mouse samples and advance their understanding of metabolic and pancreatic disorders. Order your Mouse Pancreatic Polypeptide (PP) ELISA Kit today from Assay Genie and take your research to the next level.
Product Name:
Mouse Pancreatic Polypeptide (PP) ELISA Kit
Product Code:
MODL01080
Size:
96 Assays
Target:
PP
Synonyms:
PPY, PNP, Pancreatic Polypeptide Y, Obinepitide, Pancreatic icosapeptide, Pancreatic prohormone
Detection Method:
Sandwich
Range:
31.25-2000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
