The Mouse PAP (Plasmin-Antiplasmin Complex) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of PAP levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an essential tool for researchers studying fibrinolysis and blood coagulation in mouse models.The Plasmin-Antiplasmin Complex, composed of plasmin and its inhibitor antiplasmin, is a key player in the regulation of fibrinolysis and clot formation.
Dysregulation of this complex has been implicated in various pathological conditions, including thrombosis, liver disease, and inflammatory disorders. Therefore, the Mouse PAP CLIA Kit serves as a valuable tool for investigating the role of PAP in these diseases and developing targeted therapies.Overall, the Mouse PAP CLIA Kit offers researchers a reliable and efficient method for quantifying PAP levels in mouse samples, furthering our understanding of fibrinolysis and its role in disease pathology.
Product Name:
Mouse PAP (Plasmin-Antiplasmin Complex) CLIA Kit
SKU:
MOES00478
Target:
Mouse PAP (Plasmin-Antiplasmin Complex)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse PAP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PAP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PAP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse PAP. You can calculate the concentration of Mouse PAP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-106
86-98
88-99
Average (%)
101
92
94
1:4
Range (%)
88-101
99-112
90-108
Average (%)
93
105
98
1:8
Range (%)
90-104
96-112
90-104
Average (%)
96
102
95
1:16
Range (%)
100-112
98-110
87-103
Average (%)
106
103
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-99
92
EDTA plasma (n=5)
95-109
102
Cell culture media (n=5)
87-98
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.21
3.94
19.75
2.17
3.76
18.12
Standard deviation
0.25
0.44
1.79
0.25
0.32
1.26
C V (%)
11.31
11.17
9.06
11.52
8.51
6.95
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse PAP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse PAP in samples. No significant cross-reactivity or interference between Mouse PAP and analogues was observed.
