Mouse Parathyroid Hormone Related Protein (PTHrP) ELISA Kit
The Mouse Parathyroid Hormone-related Protein (PTHrP) ELISA Kit is a powerful tool for the quantitative measurement of PTHrP levels in mouse biological samples such as serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity to ensure accurate and reproducible results for research applications related to bone metabolism, calcium regulation, and cancer biology.PTHrP is a key regulator of bone development and maintenance, as well as a critical mediator of calcium homeostasis.
Dysregulation of PTHrP expression has been implicated in various pathological conditions, including osteoporosis, hypercalcemia, and certain types of cancer. By accurately measuring PTHrP levels, researchers can gain valuable insights into the pathophysiology of these diseases and potentially identify novel therapeutic targets.
Product Name:
Mouse Parathyroid Hormone Related Protein (PTHrP) ELISA Kit
Product Code:
MODL01102
Size:
96 Assays
Target:
PTHrP
Synonyms:
PTHLH HHM PTHR PLP Osteostatin Parathyroid Hormone Like Hormone
Detection Method:
Sandwich
Range:
15.6-1,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
90-101
95
EDTA plasma (n=5)
91-99
95
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
