The Mouse PCT (Procalcitonin) CLIA Kit is specifically designed for the precise and accurate detection of procalcitonin levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring dependable and consistent results for a variety of research purposes.Procalcitonin is a key biomarker associated with bacterial infections, making it a valuable tool for diagnosing and monitoring infectious diseases in mice.
By measuring procalcitonin levels, researchers can gain insights into the severity of infections and evaluate therapeutic interventions effectively.Overall, the Mouse PCT CLIA Kit provides researchers with a reliable and efficient solution for studying infectious diseases and evaluating potential treatments in mouse models.
Product Name:
Mouse PCT (Procalcitonin) CLIA Kit
SKU:
MOES00493
Target:
Mouse PCT (Procalcitonin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse PCT. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PCT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PCT, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse PCT. You can calculate the concentration of Mouse PCT in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-99
90-104
90-104
Average (%)
93
96
96
1:4
Range (%)
85-97
95-108
89-99
Average (%)
92
101
94
1:8
Range (%)
99-115
95-107
94-109
Average (%)
106
100
101
1:16
Range (%)
93-110
99-115
101-117
Average (%)
100
105
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-112
103
EDTA plasma (n=5)
92-108
99
Cell culture media (n=5)
86-97
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.33
73.18
200.91
24.07
68.0
220.26
Standard deviation
2.04
7.77
19.81
2.13
5.9
17.84
C V (%)
8.38
10.62
9.86
8.85
8.68
8.1
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse PCT concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse PCT in samples. No significant cross-reactivity or interference between Mouse PCT and analogues was observed.
