Mouse PDGFC (Platelet Derived Growth Factor C) CLIA Kit
The Mouse PDGFC (Platelet-derived Growth Factor C) CLIA Kit is a comprehensive tool designed for the precise measurement of PDGFC levels in mouse serum, plasma, and cell culture supernatants. This kit is known for its exceptional sensitivity and specificity, ensuring accurate and consistent results for various research purposes.PDGFC is a key growth factor that plays a crucial role in promoting cell growth, proliferation, and tissue repair. It is closely associated with various biological processes, including angiogenesis and wound healing.
Studies have shown its involvement in conditions such as cancer and fibrosis, making it a valuable biomarker for investigating these diseases and developing potential therapeutic interventions.Overall, the Mouse PDGFC CLIA Kit provides researchers with a reliable and efficient tool for studying the biological functions and implications of PDGFC in different physiological and pathological contexts. Its high performance and user-friendly features make it an essential asset for any laboratory focusing on growth factor research.
Product Name:
Mouse PDGFC (Platelet Derived Growth Factor C) CLIA Kit
SKU:
MOES00483
Target:
Mouse PDGFC (Platelet Derived Growth Factor C)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse PDGFC. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PDGFC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PDGFC, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse PDGFC. You can calculate the concentration of Mouse PDGFC in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-111
99-113
89-102
Average (%)
104
105
95
1:4
Range (%)
100-114
91-107
99-112
Average (%)
108
98
106
1:8
Range (%)
104-121
99-114
89-104
Average (%)
110
107
96
1:16
Range (%)
98-109
86-98
85-97
Average (%)
103
93
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-102
93
EDTA plasma (n=5)
87-100
92
Cell culture media (n=5)
86-98
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
39.36
107.82
366.86
39.73
118.45
376.73
Standard deviation
3.92
9.47
24.91
3.48
8.59
27.31
C V (%)
9.96
8.78
6.79
8.76
7.25
7.25
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse PDGFC concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse PDGFC in samples. No significant cross-reactivity or interference between Mouse PDGFC and analogues was observed.
