The Mouse PDGFR-L (Platelet Derived Growth Factor Receptor-Like Protein) CLIA Kit is a highly sensitive and specific assay designed for the accurate measurement of PDGFR-L levels in mouse serum, plasma, and cell culture supernatants. This kit is ideal for researchers studying the role of PDGFR-L in various biological processes, including cell proliferation and angiogenesis. PDGFR-L is a key protein that plays a crucial role in cell signaling pathways, influencing cell growth, survival, and differentiation.
Dysregulation of PDGFR-L has been implicated in various diseases, including cancer and cardiovascular disorders, making it an important biomarker for disease research and therapeutic development. With its high-quality components and user-friendly protocol, the Mouse PDGFR-L CLIA Kit offers reliable and reproducible results, enabling researchers to obtain valuable data for their studies. Order your kit today and advance your research in the field of PDGFR-L biology.
Mouse PDGFRL (Platelet Derived Growth Factor Receptor Like Protein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse PDGFRL. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PDGFRL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PDGFRL, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse PDGFRL. You can calculate the concentration of Mouse PDGFRL in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-110
103-116
89-104
Average (%)
103
110
95
1:4
Range (%)
83-99
90-103
91-103
Average (%)
90
97
96
1:8
Range (%)
87-98
98-114
96-111
Average (%)
93
107
103
1:16
Range (%)
94-107
100-117
101-116
Average (%)
99
107
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-108
103
EDTA plasma (n=5)
87-100
94
Cell culture media (n=5)
93-103
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
51.47
125.29
416.48
47.93
133.29
450.79
Standard deviation
5.83
9.74
47.35
4.93
15.3
46.93
C V (%)
11.33
7.77
11.37
10.29
11.48
10.41
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse PDGFRL concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse PDGFRL in samples. No significant cross-reactivity or interference between Mouse PDGFRL and analogues was observed.
