The Mouse PECAM1 (Platelet Endothelial Cell Adhesion Molecule-1) CLIA Kit is a highly sensitive and specific assay for the accurate detection of PECAM1 levels in mouse samples, including serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it a valuable tool for research in a variety of fields.PECAM1, also known as CD31, is a key molecule involved in endothelial cell adhesion and plays a critical role in angiogenesis and vascular integrity. Dysregulation of PECAM1 has been associated with various diseases, including cardiovascular disorders, inflammation, and cancer.
Therefore, measuring PECAM1 levels can provide insights into these pathological conditions and aid in the development of potential therapeutic strategies.Overall, the Mouse PECAM1 CLIA Kit is an essential resource for researchers studying vascular biology, angiogenesis, and the role of adhesion molecules in health and disease. Its high performance and user-friendly design make it an indispensable tool for advancing scientific knowledge and uncovering novel therapeutic targets.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse PECAM1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PECAM1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PECAM1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse PECAM1. You can calculate the concentration of Mouse PECAM1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-100
94-111
97-111
Average (%)
92
101
105
1:4
Range (%)
85-101
99-115
91-104
Average (%)
92
106
97
1:8
Range (%)
101-114
101-113
96-111
Average (%)
107
107
104
1:16
Range (%)
86-97
88-103
93-104
Average (%)
92
94
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-114
106
EDTA plasma (n=5)
94-106
101
Cell culture media (n=5)
91-103
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.16
49.89
183.25
23.65
51.77
188.87
Standard deviation
1.93
4.75
11.36
2.6
5.73
18.23
C V (%)
8.33
9.52
6.2
10.99
11.07
9.65
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse PECAM1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse PECAM1 in samples. No significant cross-reactivity or interference between Mouse PECAM1 and analogues was observed.
