The Mouse PF4 (Platelet Factor 4) CLIA Kit is specifically designed for the quantitative measurement of PF4 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and consistent results for various research applications.PF4 is a key factor in platelet activation and aggregation, as well as in the immune response. It plays a crucial role in various inflammatory conditions, thrombosis, and atherosclerosis, making it a valuable biomarker for studying these processes and developing targeted treatments.
With its reliable performance and ease of use, the Mouse PF4 CLIA Kit is an essential tool for researchers investigating the role of PF4 in different physiological and pathological processes in mouse models.
Product Name:
Mouse PF4 (Platelet Factor 4) CLIA Kit
SKU:
MOES00487
Target:
Mouse PF4 (Platelet Factor 4)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse PF4/CXCL4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PF4/CXCL4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PF4/CXCL4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse PF4/CXCL4. You can calculate the concentration of Mouse PF4/CXCL4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-99
100-115
91-103
Average (%)
90
108
96
1:4
Range (%)
99-110
93-110
85-98
Average (%)
105
100
92
1:8
Range (%)
95-110
100-116
93-106
Average (%)
101
106
101
1:16
Range (%)
92-107
86-97
98-112
Average (%)
98
92
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-111
103
EDTA plasma (n=5)
92-107
100
Cell culture media (n=5)
88-101
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
46.77
101.42
425.62
44.87
101.47
439.83
Standard deviation
4.15
7.52
46.86
3.68
11.17
47.77
C V (%)
8.87
7.41
11.01
8.2
11.01
10.86
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse PF4/CXCL4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse PF4/CXCL4 in samples. No significant cross-reactivity or interference between Mouse PF4/CXCL4 and analogues was observed.
