The Mouse PF4 (Platelet Factor 4) ELISA Kit is a specialized assay designed for the quantitative detection of PF4 levels in various biological samples. PF4, a small cytokine released from platelets, plays a crucial role in modulating blood clot formation, inflammation, and the immune response. This ELISA kit enables researchers to accurately measure PF4 concentrations, facilitating investigations into its functions and implications in various biological processes. Accurate quantification of PF4 is essential for studying its involvement in platelet aggregation, thrombosis, and immune regulation.
The Mouse PF4 ELISA Kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results for your research needs. Manufactured under strict quality control standards, this kit provides reliable performance and ease of use, making it an ideal choice for investigating the role of PF4 in physiological and pathological conditions.
Product Name:
Mouse PF4 (Platelet Factor 4) ELISA Kit
SKU:
AEES00268
Target:
Mouse PF4 (Platelet Factor 4)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse PF4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PF4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PF4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse PF4. You can calculate the concentration of Mouse PF4 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-106
99-115
94-108
Average (%)
99
106
100
1:4
Range (%)
96-109
97-110
89-102
Average (%)
101
104
97
1:8
Range (%)
89-103
97-112
92-104
Average (%)
94
104
97
1:16
Range (%)
92-106
95-112
91-104
Average (%)
97
103
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-101
95
EDTA plasma (n=5)
84-99
91
Cell culture media (n=5)
84-98
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
103.27
312.54
739.29
111.53
312.5
747.32
Standard deviation
7.2
13.69
32.23
5.75
16.09
36.54
C V (%)
6.97
4.38
4.36
5.16
5.15
4.89
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse PF4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse PF4 in samples. No significant cross-reactivity or interference between Mouse PF4 and analogues was observed.
