The Mouse PHB (Prohibitin) CLIA Kit is a cutting-edge assay designed for the precise detection of prohibitin levels in mouse serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit provides consistent and accurate results, making it perfect for various research applications.Prohibitin is a key protein that plays a critical role in various cellular processes, including cell cycle regulation, apoptosis, and mitochondrial function. Dysregulation of prohibitin has been linked to various diseases such as cancer, metabolic disorders, and neurodegenerative conditions, highlighting its significance as a valuable biomarker for studying these diseases and developing potential therapeutic interventions.
This advanced CLIA kit offers researchers a reliable tool to measure prohibitin levels accurately, enabling deeper insights into its biological functions and potential implications in disease pathology. Upgrade your research capabilities with the Mouse PHB CLIA Kit and unlock new possibilities in your scientific investigations.
Product Name:
Mouse PHB (Prohibitin) CLIA Kit
SKU:
MOES00494
Target:
Mouse PHB (Prohibitin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse PHB. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PHB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PHB, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse PHB. You can calculate the concentration of Mouse PHB in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-109
92-105
101-115
Average (%)
102
99
108
1:4
Range (%)
96-109
96-110
91-108
Average (%)
103
102
99
1:8
Range (%)
91-105
93-105
84-98
Average (%)
98
100
90
1:16
Range (%)
103-117
101-117
91-106
Average (%)
109
108
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-99
92
EDTA plasma (n=5)
101-118
107
Cell culture media (n=5)
96-113
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
248.96
790.45
2085.56
271.66
818.65
2151.99
Standard deviation
22.9
59.76
127.64
34.88
77.44
129.76
C V (%)
9.2
7.56
6.12
12.84
9.46
6.03
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse PHB concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse PHB in samples. No significant cross-reactivity or interference between Mouse PHB and analogues was observed.
