SynonymsBZS,MHAM,MMAC1,PTEN1,TEP1,Mutated In Multiple Advanced Cancers 1,Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN
ReactivityMouse
Sample TypeTissue Homogenates, Cell Lysates And Other Biological Fluids
SKU:
MODL01098
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O08586
Sensitivity:
0.061ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
BZS,MHAM,MMAC1,PTEN1,TEP1,Mutated In Multiple Advanced Cancers 1,Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN
Reactivity:
Mouse
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
Mouse Phosphatase And Tensin Homolog (PTEN) ELISA Kit
*The Mouse Phosphatase and Tensin Homolog (PTEN) ELISA Kit is specifically designed for the precise measurement of PTEN levels in mouse serum, plasma, and cell culture supernatants. This kit is known for its outstanding sensitivity and specificity, ensuring reliable and consistent results that are suitable for a wide range of research applications.**PTEN is a vital tumor suppressor gene that plays a crucial role in regulating cell growth, survival, and proliferation.
Dysregulation of PTEN has been implicated in various diseases, including cancer, neurological disorders, and metabolic syndromes. Therefore, accurate measurement of PTEN levels using this ELISA kit can provide valuable insights into disease mechanisms and potential therapeutic targets.*
Product Name:
Mouse Phosphatase And Tensin Homolog (PTEN) ELISA Kit
Product Code:
MODL01098
Size:
96 Assays
Target:
PTEN
Synonyms:
BZS, MHAM, MMAC1, PTEN1, TEP1, Mutated In Multiple Advanced Cancers 1, Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
