Mouse Phospholipase A2, Group IID (PLA2G2D) ELISA Kit
The Mouse Phospholipase A2 Group IID (PLA2G2D) ELISA Kit is a powerful tool for accurately measuring levels of PLA2G2D in mouse serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.PLA2G2D is a key enzyme involved in the regulation of inflammatory responses and lipid metabolism. Dysregulation of PLA2G2D has been implicated in various diseases, including inflammatory disorders and cancer.
By accurately measuring PLA2G2D levels, researchers can gain valuable insights into the role of this enzyme in disease pathogenesis and potentially identify new therapeutic targets.Overall, the Mouse Phospholipase A2 Group IID (PLA2G2D) ELISA Kit offers researchers a comprehensive and efficient solution for studying the function and significance of PLA2G2D in mouse models, ultimately advancing our understanding of inflammatory processes and disease mechanisms.
Product Name:
Mouse Phospholipase A2, Group IID (PLA2G2D) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-98
94
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
