Mouse Phospholipase A2, Group III (PLA2G3) ELISA Kit
The Mouse Phospholipase A2 Group III (PLA2G3) ELISA Kit is designed for the accurate quantification of PLA2G3 levels in mouse serum, plasma, and tissue lysates. This kit offers high sensitivity and specificity, allowing for reliable and reproducible results suitable for a variety of research applications.Phospholipase A2 Group III is an enzyme implicated in various physiological processes, including lipid metabolism, inflammatory responses, and cell signaling. Dysregulation of PLA2G3 has been linked to inflammatory disorders, neurodegenerative diseases, and cancer, highlighting its importance as a potential biomarker for disease progression and therapeutic intervention.
With the Mouse Phospholipase A2 Group III ELISA Kit, researchers can effectively measure PLA2G3 levels in mouse samples, providing valuable insights into its role in health and disease. This kit is a valuable tool for studying PLA2G3 function, elucidating its mechanisms of action, and exploring its potential as a therapeutic target.
Product Name:
Mouse Phospholipase A2, Group III (PLA2G3) ELISA Kit
Product Code:
MODL01060
Size:
96 Assays
Target:
PLA2G3
Synonyms:
GIII-SPLA2, sPLA2-III, Group 3 secretory phospholipase A2, Group III secretory phospholipase A2, Phosphatidylcholine 2-acylhydrolase 3
Detection Method:
Sandwich
Range:
0.781-50ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
