Mouse PICP (Procollagen I C-terminal Propeptide) CLIA Kit
The Mouse PICP (Procollagen I C-terminal Propeptide) CLIA Kit is a cutting-edge assay designed for the precise measurement of PICP levels in mouse serum, plasma, and tissue culture supernatants. This advanced kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research studies.PICP is a key biomarker associated with collagen synthesis and tissue remodeling, playing a crucial role in wound healing, bone formation, and connective tissue development. Dysregulation of PICP levels has been linked to various diseases, making it an important target for the study of fibrosis, arthritis, and other inflammatory conditions.
With its user-friendly protocol and reliable performance, the Mouse PICP CLIA Kit is an invaluable tool for researchers investigating the mechanisms of collagen metabolism and its implications in health and disease. Obtain precise and reliable data with this innovative assay, opening new possibilities for understanding and targeting PICP-related pathways.
Product Name:
Mouse PICP (Procollagen I C-terminal Propeptide) CLIA Kit
SKU:
MOES00144
Target:
Mouse PICP (Procollagen I C-terminal Propeptide)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse PICP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PICP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PICP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse PICP. You can calculate the concentration of Mouse PICP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-107
101-114
101-117
Average (%)
101
108
108
1:4
Range (%)
90-104
93-107
93-106
Average (%)
98
99
100
1:8
Range (%)
97-115
85-101
94-110
Average (%)
105
92
101
1:16
Range (%)
90-106
84-100
95-111
Average (%)
97
91
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-98
92
EDTA plasma (n=5)
87-100
93
Cell culture media (n=5)
92-103
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
40.65
101.48
270.32
36.65
99.49
275.53
Standard deviation
5.08
8.79
26.63
3.04
11.38
30.91
C V (%)
12.5
8.66
9.85
8.29
11.44
11.22
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse PICP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse PICP in samples. No significant cross-reactivity or interference between Mouse PICP and analogues was observed.
