The Mouse PIIICP (C-Terminal Procollagen 3 Propeptide) CLIA Kit is a powerful tool for researchers looking to accurately measure PIIICP levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.PIIICP is a key marker for assessing collagen synthesis and degradation, providing valuable insights into tissue remodeling and fibrosis.
Monitoring PIIICP levels can offer crucial information for studying conditions such as liver disease, fibrotic disorders, and musculoskeletal diseases.With its reliable performance and broad range of applications, the Mouse PIIICP CLIA Kit is an essential tool for researchers investigating collagen metabolism and related pathologies in mouse models.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse Pâ…¢CP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse Pâ…¢CP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse Pâ…¢CP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse Pâ…¢CP. You can calculate the concentration of Mouse Pâ…¢CP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-114
102-118
97-112
Average (%)
104
109
105
1:4
Range (%)
99-115
92-106
92-105
Average (%)
105
98
99
1:8
Range (%)
101-118
95-109
98-114
Average (%)
108
102
104
1:16
Range (%)
85-99
99-113
91-104
Average (%)
92
106
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-101
96
EDTA plasma (n=5)
100-116
106
Cell culture media (n=5)
95-109
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
26.13
67.9
202.89
28.58
66.9
187.15
Standard deviation
2.78
6.23
13.47
3.32
4.71
12.26
C V (%)
10.64
9.18
6.64
11.62
7.04
6.55
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse Pâ…¢CP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse Pâ…¢CP in samples. No significant cross-reactivity or interference between Mouse Pâ…¢CP and analogues was observed.
