The Mouse PIIICP (C-terminal Procollagen 3 Propeptide) ELISA Kit is a reliable and accurate tool for measuring levels of PIIICP in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.PIIICP is an important marker for collagen synthesis and turnover, playing a key role in processes such as tissue repair and fibrosis. Monitoring PIIICP levels can provide valuable insights into collagen metabolism and various physiological and pathological conditions, including liver fibrosis, arthritis, and wound healing.
With its user-friendly protocol and high-performance features, the Mouse PIIICP ELISA Kit is an essential tool for researchers studying collagen biology and related diseases in mouse models. Trust in this kit to deliver accurate and reliable data for your research needs.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse PⅢCP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PⅢCP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PⅢCP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse PⅢCP. You can calculate the concentration of Mouse PⅢCP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-108
95-109
97-109
Average (%)
101
103
103
1:4
Range (%)
92-105
85-97
87-100
Average (%)
99
90
92
1:8
Range (%)
88-99
82-96
85-95
Average (%)
94
88
90
1:16
Range (%)
94-108
82-95
85-96
Average (%)
99
87
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-107
101
EDTA plasma (n=5)
85-95
90
Cell culture media (n=5)
87-98
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
54.69
159.79
436.26
58.02
155.01
400.77
Standard deviation
3.55
8.12
18.06
2.94
6.73
12.9
C V (%)
6.49
5.08
4.14
5.07
4.34
3.22
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse Pâ…¢CP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse Pâ…¢CP in samples. No significant cross-reactivity or interference between Mouse Pâ…¢CP and analogues was observed.
