Mouse Platelet Derived Growth Factor Receptor Like Protein (PDGFRL) ELISA Kit
The Mouse Platelet-Derived Growth Factor Receptor-Like Protein (PDGFR-L) ELISA Kit is a highly reliable and sensitive assay designed for the precise quantification of PDGFR-L levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional accuracy and reproducibility, making it an ideal tool for a variety of research applications.PDGFR-L is a key protein involved in cell growth and development, particularly in the formation of blood vessels and tissues. Its role in various physiological processes and diseases makes it a valuable biomarker for studying conditions such as cancer, cardiovascular disorders, and neurological conditions.
By accurately measuring PDGFR-L levels, researchers can gain valuable insights into disease mechanisms and potential therapeutic targets.With its high-performance capabilities and user-friendly design, the Mouse PDGFR-L ELISA Kit from AssayGenie is a valuable asset for researchers looking to advance their understanding of PDGFR-L biology and its implications in health and disease.
Product Name:
Mouse Platelet Derived Growth Factor Receptor Like Protein (PDGFRL) ELISA Kit
Product Code:
MODL01022
Size:
96 Assays
Target:
PDGFRL
Synonyms:
PRLTS, PDGF-Receptor Beta-Like Tumor Suppressor
Detection Method:
Sandwich
Range:
78.125-5000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
