The Mouse PLAU (UPA / Urokinase-type Plasminogen Activator) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of Urokinase-type Plasminogen Activator levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for various research applications.Urokinase-type Plasminogen Activator is a key enzyme involved in the regulation of the fibrinolytic system, playing a critical role in processes such as tissue remodeling, cell migration, and angiogenesis.
Dysregulation of Urokinase-type Plasminogen Activator has been implicated in various pathological conditions, including cancer, thrombosis, and inflammation, highlighting its importance as a biomarker for studying these diseases and potential therapeutic targets.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse PLAU/uPA. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PLAU/uPA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PLAU/uPA, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse PLAU/uPA. You can calculate the concentration of Mouse PLAU/uPA in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-100
89-105
85-98
Average (%)
93
96
90
1:4
Range (%)
92-105
97-111
102-116
Average (%)
99
104
109
1:8
Range (%)
95-111
85-99
89-102
Average (%)
101
91
96
1:16
Range (%)
85-96
95-109
87-100
Average (%)
91
100
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-102
95
EDTA plasma (n=5)
87-102
93
Cell culture media (n=5)
96-111
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.39
60.18
243.31
25.56
62.03
229.45
Standard deviation
3.04
5.19
17.03
2.84
6.18
17.85
C V (%)
11.97
8.62
7.0
11.11
9.96
7.78
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse PLAU/uPA concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse PLAU/uPA in samples. No significant cross-reactivity or interference between Mouse PLAU/uPA and analogues was observed.
