The Mouse POMC (Proopiomelanocortin) CLIA Kit is specifically designed for the accurate quantification of POMC levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for various research applications.POMC is a vital precursor protein that produces several important hormones, including adrenocorticotropic hormone (ACTH) and beta-endorphin. These hormones play crucial roles in regulating stress responses, inflammation, and pain perception.
Dysregulation of POMC expression has been linked to conditions such as obesity, immune disorders, and neurological diseases, making it an essential biomarker for studying these conditions and developing potential therapeutic interventions.Overall, the Mouse POMC CLIA Kit from AssayGenie offers researchers a reliable tool for investigating the role of POMC in various physiological and pathological processes in mouse models.
Product Name:
Mouse POMC (Proopiomelanocortin) CLIA Kit
SKU:
MOES00498
Target:
Mouse POMC (Proopiomelanocortin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse POMC. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse POMC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse POMC, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse POMC. You can calculate the concentration of Mouse POMC in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-104
94-107
97-112
Average (%)
99
101
103
1:4
Range (%)
101-113
98-111
102-114
Average (%)
107
105
108
1:8
Range (%)
93-105
93-107
93-105
Average (%)
100
101
99
1:16
Range (%)
87-98
94-108
87-99
Average (%)
93
100
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
102-115
108
EDTA plasma (n=5)
94-110
101
Cell culture media (n=5)
97-111
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
277.65
515.57
2099.85
279.45
466.93
1935.8
Standard deviation
29.07
44.29
192.35
33.06
33.29
144.41
C V (%)
10.47
8.59
9.16
11.83
7.13
7.46
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse POMC concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse POMC in samples. No significant cross-reactivity or interference between Mouse POMC and analogues was observed.
