The Mouse Postn (OSF-2, Periostin) CLIA Kit is a highly reliable and sensitive assay designed for the accurate detection of mouse Postn levels in various biological samples such as serum, plasma, and cell culture supernatants. This kit provides researchers with a powerful tool for studying the role of Postn in various biological processes.Postn, also known as osteoblast-specific factor 2 (OSF-2) or Periostin, is a key protein involved in tissue development and repair. It plays a crucial role in regulating cell adhesion, migration, and survival, making it an important biomarker for studying various diseases and conditions, including cancer, cardiovascular diseases, and bone disorders.
With its high sensitivity and specificity, the Mouse Postn CLIA Kit offers researchers a reliable and reproducible method for quantifying Postn levels in biological samples. This makes it an ideal tool for a wide range of research applications, including investigating the role of Postn in disease pathogenesis and identifying potential therapeutic targets.
Product Name:
Mouse POSTN/OSF-2 (Periostin) CLIA Kit
SKU:
MOES00337
Target:
Mouse POSTN/OSF-2 (Periostin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse POSTN/OSF-2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse POSTN/OSF-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse POSTN/OSF-2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse POSTN/OSF-2. You can calculate the concentration of Mouse POSTN/OSF-2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-107
101-117
103-116
Average (%)
98
109
109
1:4
Range (%)
93-106
91-108
99-115
Average (%)
98
98
107
1:8
Range (%)
86-101
86-100
88-99
Average (%)
93
93
93
1:16
Range (%)
95-110
91-104
93-109
Average (%)
103
98
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-97
91
EDTA plasma (n=5)
83-98
90
Cell culture media (n=5)
91-106
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
99.08
257.33
893.31
104.59
255.4
824.78
Standard deviation
11.54
28.0
74.68
9.03
29.27
77.86
C V (%)
11.65
10.88
8.36
8.63
11.46
9.44
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse POSTN/OSF-2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse POSTN/OSF-2 in samples. No significant cross-reactivity or interference between Mouse POSTN/OSF-2 and analogues was observed.
