The Mouse PP (Pancreatic Polypeptide) CLIA Kit is specifically designed for the quantitative measurement of pancreatic polypeptide levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.Pancreatic polypeptide is a peptide hormone produced by the pancreas that plays a critical role in regulating pancreatic exocrine and endocrine functions.
Abnormal levels of pancreatic polypeptide have been associated with various metabolic disorders, making it a valuable biomarker for studying these conditions and developing potential treatments.With its easy-to-use format and reliable performance, the Mouse PP CLIA Kit from Assaygenie is an essential tool for researchers investigating the role of pancreatic polypeptide in metabolic diseases and exploring new therapeutic strategies.
Product Name:
Mouse PP (Pancreatic Polypeptide) CLIA Kit
SKU:
MOES00466
Target:
Mouse PP (Pancreatic Polypeptide)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse PP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse PP. You can calculate the concentration of Mouse PP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-103
86-98
84-95
Average (%)
98
93
90
1:4
Range (%)
89-99
92-106
104-119
Average (%)
94
99
110
1:8
Range (%)
96-108
90-105
92-104
Average (%)
102
96
98
1:16
Range (%)
87-99
89-102
85-100
Average (%)
94
95
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-104
95
EDTA plasma (n=5)
99-114
107
Cell culture media (n=5)
85-97
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
41.66
105.92
331.77
38.8
116.33
310.49
Standard deviation
3.38
12.09
23.16
3.51
10.26
23.72
C V (%)
8.11
11.41
6.98
9.05
8.82
7.64
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse PP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse PP in samples. No significant cross-reactivity or interference between Mouse PP and analogues was observed.
