The Mouse PRL (Prolactin) CLIA Kit is a reliable and accurate tool for measuring prolactin levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, providing researchers with dependable and consistent results for a variety of research applications.Prolactin is a hormone with diverse functions, including regulating lactation, fertility, and immune response. Changes in prolactin levels have been linked to various physiological and pathological conditions, making it an important biomarker for studying reproductive health, immune function, and endocrine disorders.
With its ease of use and robust performance, the Mouse PRL CLIA Kit is a valuable resource for researchers looking to explore the role of prolactin in biological processes and disease. Trust Assay Genie for high-quality kits that deliver precise and reliable data for your research needs.
Product Name:
Mouse PRL (Prolactin) CLIA Kit
SKU:
MOES00063
Target:
Mouse PRL (Prolactin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse PRL. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PRL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PRL, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse PRL. You can calculate the concentration of Mouse PRL in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-100
91-105
91-107
Average (%)
93
99
99
1:4
Range (%)
94-112
89-104
100-119
Average (%)
102
95
108
1:8
Range (%)
93-107
83-95
87-102
Average (%)
100
90
94
1:16
Range (%)
100-114
96-110
94-106
Average (%)
108
103
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-101
95
EDTA plasma (n=5)
99-116
107
Cell culture media (n=5)
86-98
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
104.9
303.11
732.76
112.34
317.73
784.07
Standard deviation
8.51
33.49
80.6
10.72
32.92
56.92
C V (%)
8.11
11.05
11.0
9.54
10.36
7.26
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse PRL concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse PRL in samples. No significant cross-reactivity or interference between Mouse PRL and analogues was observed.
